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Thermo fisher scientific live/dead cell viability assays are designed for easy and sensitive differentiation of live, dead, and cytotoxic populations using fluorescence microscopy, flow cytometry, or microplate readers Staining concentration, incubation time, and the. Live/dead assay live/dead assays often rely upon both the membrane integrity and either esterase or metabolic activity of live populations and involve the use of two dyes, one that stains live populations, and one that stain dead populations
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The dead cell dye is generally membrane impermeant and therefore cannot pass through intact cell membranes. Cited in > 50 publications. Live cell imaging is the study of cellular structure and function in living cells via microscopy
It enables the visualization and quantitation of dynamic cellular processes in real time
The ability to study cellular and subcellular structure, function, and organization in living systems aids in the development of assays that are more biologically relevant and that can better predict the. Use with flow cytometer and fluorescence microscope
